||The microsphere beads are used in biomedical applications due to their ability to facilitate procedures such as cell sorting and immunio precipitation. Novel organic dyes can use to chemical sensors, biosensing materials and then we makes organic dyes that have aldehyde functional group with fluorescent materials. Then, This organic dye was encapsulated in Poly(methyl methacrylate) (PMMA) microsphere beads. PMMA is an important polymeric material with high light transmittancy, colorlessness, chemical resistance, and weathering corrosion resistance. Due to these characteristics, PMMA was suspension-polymerized with methyl methacrylate(MMA) and ethylene glycol dimethacrylate(EGDMA) that is crosslinked microsphere bead.