화학공학소재연구정보센터
학회 한국화학공학회
학술대회 2010년 가을 (10/21 ~ 10/22, 대전컨벤션센터)
권호 16권 2호, p.1594
발표분야 생물화공
제목 Immobilization of cysteine-tagged multimer-protein G on amine functionalizedFe3O4@SiO2
초록 This work reportsthe immobilization ofmonomer, dimer, trimer, tetramer (multimer) protein G onto amine functionalized magnetic silica nanoparticles for self-orientation and immobilization of antibody. The surface of the magnetic silica nanoparticles was modified with 3-aminopropyl-trimethoxysilane (APTMS) to chemically link to multimer protein G. The conjugation of amine on the MNPs to cysteine tag in multimer protein Gs was performed using a sulfo-SMCC coupling procedure. The binding efficiencies of mono-, di-, tri- and tetramer were 77%, 51%, 43%, 20% respectively. The bound of multimer protein Gs was characterized by using ATR FT-IR, and transmission electron microscopy (TEM), and UV-VIS spectrophotometer test. Afterward mouse IgG, model antibody, was immobilized on the nanoparticle through multimer protein Gs. The multimer protein Gs bound magnetic silica nanoparticles was found to be a more suitable tool for a sensitive immunosensor.
저자 최홍경1, 안치영1, 이진형2, 김중배3, 장정호2
소속 1한국세라믹기술원 / 고려대, 2한국세라믹기술원, 3고려대
키워드 magnetic silica nanoparticle; self-orientation; antibody immobilization; immunoassay
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