||Silver nanoparticle embedded poly(vinyl alcohol)-poly(methyl methacrylate) nanofibers were fabricated by one-step radical mediated dispersion polymerization using 2,2’-azobis(isobutyronitrile) as a reductant and initiator. In this methodology, PVA acted both as a gelator to form the nanofibers and as a stabilizer to protect the silver clusters from sintering. UV-vis spectroscopic analysis indicated that the silver nanoparticles were continually released from the polymer nanofiber in aqueous solution. The antimicrobial properties of silver/PMMA nanofiber against both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria were evaluated using minimum inhibitory concentration (MIC), modified Kirby-Bauer method, and kinetic test. The MIC test demonstrated that the silver/PMMA nanofiber had the enhanced antimicrobial efficacy than silver sulfadiazine and silver nitrate at the same silver concentration.