||Fiberization of two-dimensional (2D) materials have been considered as an essential strategy for realizing their excellent chemical and mechanical properties at macroscale by controlling microstructure. Ti3C2Tx MXene is a newly discovered 2D nanomaterial with high electroconductivity and mechanical properties. Herein, we succeeded in fabricating pure MXene fibers via a large-scale wet-spinning assembly. MXene dispersion was gelated by ammonium cations which reinforces electrostatic attractions between MXene sheets. During the wet spinning process, MXene dispersion was transformed into a structural aligned fiber under mechanical drawing force in coagulate bath, because the gel can endure structural deformation. Oriented MXene fibers exhibit a strong tensile strength of 344 MPa and high electrical conductivity of 12 504 S cm–1, which is 5 times higher than that of MXene film. The obtained MXene fiber was applied to flexible electrical wires for operating keyboard, ear phone, and LEDs.