||pH-sensitive polymers are used for many applications such as drug delivery, gene delivery, especially, cancer treatment. Because tumor microenvironment (TME) and lysosome have each acidic pH 6.8 and pH 5.5 unlike normal cells (pH 7.2 ~7.4). But, pH-sensitive polymers have some demerits such as certainly necessary synthesis, wide distribution of molecular weight, difficult to regulate toxicity and biodegradation, drug loading efficiency and release rate. C-Dots are composed organic materials containing carbon. C-Dots have many advantages that fluorescence, low cost, excellent biocompatibility easy synthesis such as hydrothermal, microwave, etc. We develop TME and lysosome pH-sensitive C-Dots (TLpSC) through microwave method. Drug release in TLpSC are higher than in control group at TME. In vitro, we confirmed the therapeutic effect using various cancer cell lines. In vivo, we confirmed the tumor treatment ability of drug loading TLpSC using tumor bearing mouse.