화학공학소재연구정보센터
학회 한국공업화학회
학술대회 2020년 가을 (10/28 ~ 10/30, 광주 김대중컨벤션센터(Kimdaejung Convention Center))
권호 24권 1호
발표분야 포스터-생체재료
제목 Human mesenchymal stem cells modification via photosensitizer to secret ligand inducing tumor targeted cell death
초록 In gene therapy, the delivery of gene to the target site is the most important. However, the gene therapy has a disadvantage, off-target effect, of causing inordinate biodistribution to major organs. The off-target effect can cause side effect such as normal cell death and decrease therapeutic effect. To complement for drawback, human mesenchymal stem cells (hMSCs) were used because hMSCs can migrate to tumor site by homing effect. Also, we used polymer as non-viral vector and the polymer was complexed with gene (g/plex). The gene expresses tumor targeted cell death ligands that kills tumor cells through apoptosis. However, the transfection efficiency of the g/plex was lower than viral vector. Therefore, we utilized a photosensitizer to enhanced efficiency of the g/plex transfection into hMSCs. Overall, cell death ligand expressing gene was successfully transfected into hMSCs via complexation and photosensitizer, so that hMSCs were modified to secrete cell death ligands at tumor site.
저자 박소연, 한지은, 황희숙, 나건
소속 가톨릭대
키워드 gene delivery; gene therapy; non-viral vector; human mesenchymal stem cell; photosensitizer
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