||A sonodynamic therapy (SDT) has been investigated as a cancer treatment using ultrasound (US) mediated reactive oxygen species (ROS) generation. However, there is a limit to the development of clinically applied sonosensitizers due to the low ROS quantum yield. In this study, we developed a nanosensitizer (NS) with perfluorocarbon (PFC) to amplify ROS generation with bubble cavitation by US irradiation. The PFC loaded nanosensitizers (PNSs) generated a significant amount of ROS in vitro and in vivo compared to the bare NSs. As a result, PNSs have effectively inhibited the tumor growth due to cavitation-induced ROS generation under US irradiation. Furthermore, due to the vaporization of the PFC, a strong photoacoustic (PA) signal was generated up to 6h. In summary, PNSs has a great potential as a theranostic agent for cancer treatment.