화학공학소재연구정보센터
학회 한국화학공학회
학술대회 2020년 가을 (10/14 ~ 10/16, e-컨퍼런스)
권호 26권 1호, p.341
발표분야 생물화공
제목 Environmentally-friendly chitosan nanoparticles with GOx as highly-effective antimicrobial agents
초록 Enzymes can be used as an environmentally friendly alternative to conventional antimicrobials. Glucose oxidase (GOx), hydrogen peroxide (H2O2) generating enzyme, is a prime example. Here, we have demonstrated GOx, immobilized chitosan nanoparticles (CS-NPs) for highly effective antimicrobial approach. CS-NPs were prepared, followed by the immobilization of GOx via four approaches of covalent attachment (CA), enzyme coating (EC), enzyme precipitate coating (EPC), and magnetic nanoparticle-incorporated EPC (Mag-EPC). EPC represents an approach consisting of enzyme covalent attachment, precipitation, and cross-linking, with CA and EC being control samples while Mag-EPC was prepared by mixing magnetic nanoparticles with enzymes during the preparation of EPC. Activity of Mag-EPC was about 26-fold higher than that of the CA and 12-fold higher than the EC. Also, EPC and Mag-EPC have 3-fold higher stability than CA and EC. In the presence of glucose, 100% bacterial killing against S. aureus was observed in EPC and Mag-EPC after the incubation, while there is no significant bacterial killing with the controls, CA, and EC. This opens up potential applications in many different fields.
저자 김리나1, 연경민2, 유지성1, Manab Deb Adhikari1, 권석준3, 김문일4, Jonathan S. Dordick3, 김중배1
소속 1고려대, 2삼성물산, 3Rensselaer Polytechnic Institute, 4가천대
키워드 나노바이오
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