|학술대회||2019년 가을 (10/30 ~ 11/01, 제주국제컨벤션센터(ICC JEJU))|
|제목||Label-Free SERS Selective Detection of β-Lactamases Using Solid Phase Microextraction|
|초록||β-lactamase is an important bacterial enzyme with the ability to efficiently cleave β-lactam antibiotics. Therefore, there are reports of unscrupulous manufacturers illegally adding β-lactamase into milk to hide antibiotic residues. It is important to develop a reliable and highly sensitive on-site and real-time detection of β-lactamase in dairy products. Surface-enhanced Raman spectroscopy (SERS) is a highly sensitive, fingerprint-based, fast analysis technique. We combined solid phase microextraction (SPME) and SERS technique to develop a rapid and highly sensitive detection method for β-lactamase in dairy products.
In this work, Ag-loaded TiO2 nanotube arrays fiber exhibited remarkable SERS activity and SPME selectivity. The results were shown that β-lactamase in milk solution at a low concentration of 0.5U/L can be easily detected without any pretreatment.
|저자||Zhu Mao1, Mengyu Jing2, 정영미1|
|소속||1강원대, 2Changchun Univ. of Technology|
|키워드||SERS; label-free; detection; TiO2 nanotube|