||Poly(vinyl alcohol) (PVA), a water-soluble biodegradable polymer, has been receiving numerous attention in packaging application. However, PVA is restricted by its low mechanical strength and poor barrier properties. Graphene have also been considered as promising nanoscale fillers in gas barrier application of polymer nanocomposites. Moreover, it increases mechanical properties. With the presence of nanoplatelets in a polymer matrix, the barrier properties of nanocomposites are expected to be significantly enhanced, mainly attributing to torturous path effect for diffusing molecules. Cellulose nanofiber (CNF) is an ideal reinforcing agent for fabricating PVA-based composites with obtaining a uniform dispersion of CNF in the polymer matrix and strong CNF/PVA interaction. In the present work, graphene oxide(GO) and CNF were combined in two differentiations and incorporated into PVA. The oxygen transmission rate of PVA/GO/CNF nanocomposite decreased more than Neat PVA.