화학공학소재연구정보센터
학회 한국고분자학회
학술대회 2019년 가을 (10/09 ~ 10/11, 제주컨벤션센터)
권호 44권 2호
발표분야 의료용 고분자 부문위원회 II
제목 Cyto-compatible Hyaluronate-hydroxyethyl Acrylate-gelatin Methacryloyl Terpolymeric Hydrogel for Cartilage Tissue Engineering and Drug Delivery Applications
초록 The present work investigates the properties of terpolymeric hydrogel of hyaluronate (HA) as a cyto-compatible biomaterial with high mechanical properties via conventional free radical polymerization technique. To improve the mechanical properties, 2-hydroxyethyl acrylate (HEA) was attached in HA, while to enhance the cell attachment and proliferation, gelatin methacryloyl (GM) was used as a cross-linker. The chemical modifications were characterized using FTIR and 1H-NMR, whereas the thermal and mechanical properties were investigated using TGA and texture analysis, respectively. The surface morphology of the dried hydrogels was observed under SEM which showed highly interconnected porous structures suitable for cell attachment and proliferation. The cytotoxicity effects of the produced hydrogels were tested in vitro using chondrocytes cells through common cytotoxicity assays. Further in vivo immunogenic response of the hydrogels was studied in a mouse model by implanting subcutaneously. Also, the ability of the hydrogels to load and release different proteins and drugs in a sustained way was demonstrated using a model protein and drug, bovine serum albumin and corticosteroid drug-dexamethasone, respectively at normal physiological conditions in vitro. On whole, the exceptional mechanical properties, interconnected porous structure, stability, biocompatibility, sustained release properties of the HA-g-pHEA-x-GM hydrogel shows its high applicability in cartilage tissue engineering and drug delivery. (1) Das et al., Carbohydr. Polym., 207, 628 (2019).
저자 Dipankar Das1, 조하나1, 김나혜1, Thi Thu Hien Pham1, 김인걸2, 정은재2, Gopinathan Janarthanan1, 노인섭1
소속 1서울과학기술대, 2서울대
키워드 Tissue engineering; Terpolymer; Hydrogel
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