||Glutaric acid is a dicarboxylic acid that is used as a platform chemical for the production of plastics and plasticizers. Currently, glutaric acid is produced through a chemical process that involves toxic compounds like potassium cyanide. In this aspect, the use of bio-based microbial fermentation is a better sustainable and eco-friendly alternative process for glutaric acid production. In this presentation, we report the development of a recombinant C. glutamicum strain for the production of glutaric acid from glucose via 5-aminovalerate (5-AVA) and cadaverine pathway. To establish glutaric acid production via 5-AVA pathway, 5-aminovalerate transaminase, glutarate semialdehyde dehydrogenase, lysine 2-monooxygenase and δ-aminovaleramidase was expressed in recombinant C. glutamicum. For glutaric acid production via cadaverine pathway, 5-aminovalerate transaminase, glutarate semialdehyde dehydrogenase, putrescine transaminase, gamma-aminobutyraldehyde dehydrogenase and putrescine importer were expressed in recombinant C. glutamicum. The detailed strategies and results will be presented in this presentation.