화학공학소재연구정보센터
학회 한국고분자학회
학술대회 2018년 가을 (10/10 ~ 10/12, 경주컨벤션센터)
권호 43권 2호
발표분야 의료용 고분자 부문위원회 II
제목 Evaluation of nanoparticles based on biocompatible glycol chitosan as a gene delivery carrier
초록 Since chitosan is biocompatible and biodegradable, it has been studied as a good material as a gene delivery carrier. In this study, we have developed and studied a gene delivery carrier by introducing methyl acrylate and polyethylenimine using glycol chitosan, a derivative of chitosan. GMP1300 and GMP2000 were identified by 1H-NMR and electrophoresis was performed to confirm the binding ability with pDNA. The particle size and surface charge of GMP1300 and GMP2000 with pDNA were measured using a zetasizer. Cytotoxicity was assessed using HeLa and HEK293 cell lines, and cellular uptake was confirmed by confocal microscopy. In addition, the gene expression rate in HeLa cell line was confirmed by confocal image through GFP, and transfection efficiency was measured by protein quantification. As a result, the prepared nanoparticles are expected to be used as biocompatible gene delivery carriers because of their low toxicity and strong binding force with genes.
저자 이영화, 최준식
소속 충남대
키워드 gene delivery; biocompatibility; glycol chitosan; polyethyleneimine; transfection
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