||Silk fibroin(SF) has importance to biomedical engineering due to its biocompatibility and low immunogenicity. Among various fabricated forms such as fibers, films, and beads, hydrogel forms are especially useful to mimic the properties of the extracellular matrix. In order to overcome the limitation caused by the brittle behavior of β-sheet in silk proteins, chemical cross-linking of tyrosine using horseradish peroxidase(HRP) and hydrogen peroxide(H2O2) becomes an attractive method to create SF hydrogels; however, the toxicity of H2O2 is still concerned. Here, we prepared SF hydrogels triggered by glucose and glucose oxidase(GOx) to avoid the above problems. Therefore, chemically cross-linked SF hydrogels with low toxicity is obtained. The gelation behavior of SF hydrogels varying the glucose concentration was investigated, and structural analysis was performed by FE-SEM and FT-IR. Furthermore, the stability of SF hydrogels was identified by several experiments including UTM.