||Polybutylene succinate (PBS) is a bio-polyester which has better processability and different mechanical properties with high heat resistant compared to other bio polymers and also decompose into water and carbon dioxide with the microorganisms under the soil. In this study, bio-PBS was electrospun into the 3D nanofibrous structure by self-modified electrospinning machine. In order to find the optimal electrospinning conditions, parameters and solution properties of bio-PBS, a series of experiments were conducted. Modifying electrospinning with liquid bath support system, we achieved randomly organized 3D nanofibrous structure. The structure and properties of the nanofibrous scaffold were studied by SEM, DSC, FTIR and XRD. Wettability by water contact angle measurement and in vitro degradation were studied for 2D as well as 3D nanofibrous scaffolds. Our study encourages utilizing bio-PBS based nanofibrous scaffolds have a great degree in biomimetic structures.