화학공학소재연구정보센터
학회 한국화학공학회
학술대회 2019년 봄 (04/24 ~ 04/26, 제주국제컨벤션센터)
권호 25권 1호, p.31
발표분야 고분자(Polymer)
제목 Immobilization of biomacromolecules on poly(pentafluorophenyl acrylate) grafted surfaces
초록 Reactive pentafluorophenyl acrylate (PFPA) polymer grafted substrate surfaces can be utilized for immobilization of biomacromolecules.  In this study, we demonstrate a method to prepare poly(PFPA) grafted surfaces via a simple two-step process.  In the first step, 3-aminopropyltriethoxysilane (APTES) is deposited as a linker molecule onto the SiO2 surface.  In the second step, poly(PFPA) homopolymer, synthesized via the reversible addition and fragmentation chain transfer (RAFT) polymerization, is grafted to the linker molecule through the exchange reaction between the pentafluorophenyl (PFP) units on the polymer and the amine groups on APTES.  Prior to biomolecule immobilization, control of surface hydrophilicity of the polymer grafted substrate is also important.  By partial substitution of the PFP units with various hydrophilic molecules, we show that a balance between desired surface hydrophilicity and number of free PFP units available for immobilization reaction can be achieved.  The optimized poly(PFPA)-grafted substrate surface is immobilized with antibody and successfully utilized in the detection of a range of biomolecules, including proteins, DNAs, and RNAs.
저자 김수라, Li Sheng
소속 한국과학기술원
키워드 고분자
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