화학공학소재연구정보센터
학회 한국화학공학회
학술대회 2018년 가을 (10/24 ~ 10/26, 대구 EXCO)
권호 24권 2호, p.1464
발표분야 생물화공(Biochemical Engineering)
제목 sRNA-based knock-down system for Biosynthesis of Phenol from Glucose in Escherichia coli
초록 Phenol is an industrially attractive and commonly used chemical, which is produced from fossil resources mostly. Previously, phenol’s biological production from renewable resources has been limited due to its toxicity to microorganisms. Here, we simultaneously engineered 18 Escherichia coli strains for the production of phenol by employing the strategy of synthetic regulatory sRNA technology. sRNA-based knock-down of the two regulators and overexpression of the genes involved in the tyrosine biosynthetic pathway together with tyrosine phenol-lyase in E. coli strains resulted in the production of phenol from glucose. Among the engineered E. coli strains, the BL21 strain produced phenol most efficiently: 419 mg/L by flask culture and 1.69 g/L by fed-batch culture. The final titer and productivity were further improved through biphasic fed-batch fermentation using glycerol tributyrate as an extractant of phenol. The concentration of phenol in the glycerol tributyrate phase and fermentation broth reached 9.84 and 0.3 g/L, respectively, in 21 h, which translates into the final phenol titer and productivity of 3.79 g/L and 0.18 g/L/h, respectively.
저자 김지용, 김병진, 박혜권, 나도균, 이상엽
소속 한국과학기술원
키워드 생물화공
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