화학공학소재연구정보센터
학회 한국공업화학회
학술대회 2018년 봄 (05/02 ~ 05/04, 대구 엑스코(EXCO))
권호 22권 1호
발표분야 우수논문(구두)-박사과정
제목 Development of a real-time permeability assay for drug molecules through a planar phospholipid bilayer
초록 Cell membrane permeability is one of the crucial parameters in the pharmaceutical industry when designing a new drug. Thus, various in vitro assays have been developed to estimate cell membrane permeability of the drug. Instead of a real cell membrane, many assays use an artificial membrane whose microscopic structures are usually unknown, requiring a more accurate and versatile technique. In this research, we developed a new platform to measure the permeability of drug molecules across a planar phospholipid bilayer with a well-defined area and structure. The entire system was constructed within a conventional UV spectrometer cell, and the transport of drug molecules across the bilayer was recorded by UV absorbance in real time. We obtained a much higher permeability compared to the other assays, and this is related to the thickness of the lipid bilayer. Also, we could observe the real-time permeability changes upon the addition of a membrane-disrupting surfactant.  
저자 이요한, 이현로, 김규한, 최시영
소속 한국과학기술원(KAIST)
키워드 Lipid bilayer; artificial cell membrane; membrane permeability; UV spectroscopy; real-time analysis
E-Mail