화학공학소재연구정보센터
학회 한국공업화학회
학술대회 2017년 봄 (05/10 ~ 05/12, 광주 김대중컨벤센센터(Kimdaejung Convention Center))
권호 21권 1호
발표분야 나노
제목 Nanoplasmonic system of RNA folding and assembly during pre-mRNA splicing
초록 RNA splicing plays important roles in transcriptome and proteome diversity. Herein, we describe the use of a nanoplasmonic system that unveils RNA folding and assembly during pre-mRNA splicing wherein the quantification of mRNA splice variants is not taken into account. With a couple of SERS-probes and plasmonic probes binding at the boundary sites of exon-2/intron-2 and intron-2/exon-3 of the pre-mature RNA of the β-globin gene, the splicing process brings the probes into the plasmonic bands. For plasmonic probes, a plasmon shift increase of ∼29 nm is measured by plasmonic coupling. The increased scattering intensity and SERS fingerprinting reveal the clear dynamics of premRNA splicing. Moreover, a time-resolved experiment of individual RNA molecules exhibited a successful splicing and an inhibited splicing event by 33 μM biflavonoid isoginkgetin, a general inhibitor of RNA splicing. The results show that the RNA splicing is successfully monitored with the nanoplasmonic system. 
  
저자 유병선, 송소진, Anh H. Nguyen, 심상준
소속 고려대
키워드 RNA splicing; Nanoplasmonic system; Plasmonic coupling; Raman scattering (SERS)
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