화학공학소재연구정보센터
학회 한국공업화학회
학술대회 2016년 가을 (10/26 ~ 10/28, 제주국제컨벤션센터(ICCJEJU))
권호 20권 2호
발표분야 나노_포스터
제목 Nanoplasmonic biosensors for detection of circulating tumor DNA(ctDNA) in genetic and epigenetic condition
초록 Circulating tumor DNA (ctDNA) are promising biomarker for noninvasive cancer assay in tumor-specific mutation and methylation. However, previous methods for ctDNA detection have limitation in genetic mutations. Here we present a strategy for ultrasensitive detection of tumor-specific mutations and methylation of ctDNA based on localized surface plasmon resonance (LSPR) and the coupling plasmon mode of gold nanoparticles (AuNPs). Peptide nucleic acid (PNA) is used as a probe to capture and enrich ctDNA. The exposure of PNA-probed AuNPs to target ctDNA generates the primary response of LSPR-peak shift. Immunogold colloids are exploited as methylation detectors and plasmon coupling enhancers for secondary response. LSPR-peak shift is increased approximately 107% upon immunogold colloids binding to two methylcytosines, compared to the primary response. These results demonstrate that the sensor can simultaneously detect the hot-spot mutation and epigenetic changes on the ctDNA.
저자 김우현, nguyenhunganh, 심상준
소속 고려대
키워드 circulating tumor DNA; mutation; DNA methylation; localized surface plasmon resonance (LSPR)
E-Mail