화학공학소재연구정보센터
학회 한국고분자학회
학술대회 2016년 봄 (04/06 ~ 04/08, 대전컨벤션센터)
권호 41권 1호
발표분야 의료용 고분자 부문위원회 I
제목 Polysaccharide-Based Viscous Cell Culture Matrix for Stem Cell Proliferation
초록 Stem cells with the capacity to self–renew and differentiate into specific cell types have been considered as a promising cell source for tissue engineering and disease modeling. However, development of cell culture system for reliable, reproducible, scalable production of the stem cells is remained as a challenge for scientists. In recent, a variety of cell culture systems based on embryonic fibroblast feeder, matrigel, hyaluronic acid are adapted for stem cell proliferation. However, the contamination by animal-derived cell/pathogen transmission, difficult cell harvesting are considered as drawbacks for clinical applications. In this study, we developed a 3D cell culture system consisted of viscous hyaluronic acid(HA) solution and porous membrane with selective permeability(permeation of nutrients/O2, but prevention of HA leakage) for stem cell proliferation. The morphology of membrane, permeability test, and cell proliferation behavior in the 3D culture system were investigated.
저자 안보슬1, 이진호2, 오세행1
소속 1단국대, 2한남대
키워드 HA; 3Dcellculture; stemcell
E-Mail