|학술대회||2004년 봄 (04/09 ~ 04/10, 고려대학교)|
|권호||29권 1호, p.335|
|발표분야||의료용 고분자 부문위원회|
|제목||Bone formation of Muscle drived stem cells in ipriflavone impregnated PLGA scaffold|
|초록||Current tissue engineering techniques require the use of a porous biodegradable/bioresorbable scaffold, which serves as three-dimensional template for initial cell attachment and subsequent tissue formation, both in vitro and in vivo. Ipriflavone (IP), a non-hormonal isoflavone derivative, has been shown to interfere with bone remodeling by inhibiting bone resorption and stimulating bone formation. IP-loaded PLGA scaffolds were prepared by solvent casting/particle leaching. Characterizations of IP contained PLGA scaffold were analyed by SEM, mercury porosimeter, and so on. Muscle-derived stem cells (MDSCs) can be differentiated in culture into osteoblasts, chondrocytes, and even myoblasts. Cellular viability and proliferation were assayed by MTT test. Osteogenic differential cell were analyzed by alkaline phosphate activity, and RT-PCR. IP/PLGA scaffolds were implanted into the back of athymic nude mouse to observe the effect of IP on the osteoinduction compared with control PLGA scaffolds. Thin sections were cut from paraffin embedded tissues and histological sections were stained H&E, von Kossa, and immunohistochemical staining for TypeⅠcollagen and osteocalcin.
This work was supported by MOWH (IMT-2000, 01-PJ11-PG9-01NT00-0011).
|저자||장지욱1, 양대혁2, 김은정2, 이봉1, 한창환3, 김문석4, 조선행4, 이해방4, 강길선2|
|소속||1부경대, 2전북대, 3가톨릭의과대, 4한국화학(연)|
|키워드||Bone formation; Ipriflavone; MDSCs; scaffold; osteoinduction|