|학술대회||2006년 가을 (11/03 ~ 11/03, 수원대학교)|
|발표분야||제11회 신소재 심포지엄|
|제목||Bio-Functionalization of Superparamagnetic Iron-Oxide Nanoparticles for Protein Separation/Purification|
|초록||The dependence of physical properties of materials on particle size is a well-known phenomenon. The interest in this field is increasing since materials with nanometer-size particles (1-100nm in diameter) exhibit novel electronic, optical, magnetic and chemical properties. Among these fields, especially, application of nano-sized magnetic particle for bio separation process has been paid many attentions in biology and biotechnology as it has higher potential for magnetic carrier. In spite of increasing popularity in biotech applications, however, few successful attempts to scale up magnetic separations in biotechnology have been made. The superparamagnetic behavior of magnetic nanoparticles for use in protein separation and purification process is of interest because they do not retain any magnetism after removing the magnetic field. Also, they have to be anti-toxic and stable in aqueous matrices. Several available approaches to magnetic separation are to synthesize magnetic iron oxide nanoparticle dispersed SiO2 nanocomposite particle, because the SiO2 materials easily formed bioactive radicals on their surface which can be modifying the cross linker for attaching the protein or antibody on the magnetic nanoparticles. Also, MgO and Au as a coating materials were a candidate materials for this application.
In this study, superparamagnetic gamma-Fe2O3 and Fe3O4 nanoparticles modified by inorganic MgO, SiO2 and Au and organic meso-2,3-Dimercapto-succinic acid(DMSA) and 3-thiopheneacetic acid(3TA) by aerosol process (such as the spray pyrolysis and electro-hydrodynamic spray processes) and/or modified chemical process.
The comparison/analysis of each process was characterized by using X-ray diffractometry, BET, SEM, TEM, FT-IR spectrometry. And magnetic properties have been studied from the hysteresis loops using a VSM and SQIUD measurements.
The magnetic behavior was drastically changed by the particle size and the synthesized nanocomposites consisted of iron-oxide phases having the finer sizes of 10 nm level show the superparamagnetism. Also, from the FT-IR measurement, the bioactive radicals caused by coating materials were indicated for the given nanoparticles, and using these functionalized nanoparticles, it was applicable for the protein (such as Lysozyme and Glutathione) separation/ purification and the localized hyperthermia.
|저자||좌용호, 김민정, 양재교, 김기도|
|키워드||초상자성 나노입자; 단백질 분리/정제; 표면수식|