화학공학소재연구정보센터
Journal of Bioscience and Bioengineering, Vol.114, No.1, 110-112, 2012
In vitro selection of bacteriophage promoters employing a terminally capped template DNA and a streptavidin-binding aptamer
In vitro selection system for the isolation of bacteriophage promoters was developed. Employing the system, one round of the selection achieved similar to 20-30 fold enrichment of an active promoter from mixtures with an inactive one. In addition, active T7 promoter variants were successfully selected from a library with six randomized bases. (C) 2012, The Society for Biotechnology, Japan. All rights reserved.