화학공학소재연구정보센터
Current Microbiology, Vol.65, No.3, 254-261, 2012
Helicobacter pylori HP0876 is Dispensable for Heme-Iron Acquisition but Attenuates Bacterial Adherence to Gastric Epithelial Cells
Helicobacter pylori can efficiently capture iron either from free heme or heme-containing compounds in the iron-limited gastric mucosa. However, the heme iron utilization systems of H. pylori have not been fully described to date. To investigate the contribution of genes involved in heme-iron utilization, a gene homologous to frpB, encode by hp0876 in H. pylori ATCC 26695, was inactivated by homologous recombination. Delta hp0876 showed no demonstrable growth defects in the presence of the various concentrations of free iron. Moreover, when hemoglobin or heme was supplied as the sole iron sources, Delta hp0876 had growth curves similar to the wild-type strain. The growth competition experiments in vitro also showed that Delta hp0876 retained the ability for iron acquisition. Furthermore, IL-8 production in human gastric epithelial cells co-cultured with Delta hp0876 and wild-type strain was compared, and our results indicated that lack of HP0876 affected the IL-8 release. And Delta hp0876 exhibited significantly increased adherence to gastric epithelial cells. Together, our data suggests that HP0876 is dispensable for H. pylori heme-iron uptake, but it may attenuate H. pylori adherence to gastric epithelial cells, which induced decreased production of H. pylori-induced IL-8 production in gastric epithelial cells.