화학공학소재연구정보센터
Current Microbiology, Vol.64, No.3, 234-241, 2012
SigB-Dependent Tolerance to Protein Synthesis-Inhibiting Antibiotics in Listeria monocytogenes EGDe
The alternative sigma factor SigB in food-borne pathogen Listeria monocytogenes was determined in this study to be required for tolerance to protein synthesis-inhibiting antibiotics. The minimum inhibitory concentrations of tetracycline HCl and gentamicin sulphate against EGDe Delta sigB were two- and fourfold less than those for EGDe, respectively. The ability of EGDe Delta sigB to overcome the growth arrest caused by erythromycin and rifampin was also weaker than that of EGDe. The transcription analysis of four genetic loci (known to be induced by rifampin in Bacillus subtili) kat, fri, ropB and rsbU in EGDe and EGDe Delta sigB in the absence or presence of rifampin revealed that: (1) expression of kat and fri genes is sigma(B) dependent, but only the former is inducible by rifampin stress; (2) the transcriptional level of rpoB gene was stable under all the experimental conditions, while that of rsbU in EGDe Delta sigB was remarkably higher in the absence of rifampin and significantly increased in EGDe but reduced in EGDe Delta sigB after rifampin application, when compared to those in EGDe and EGDe Delta sigB control without antibiotic, respectively. These results suggest that complex physiological reactions to tolerance of the antibiotic stress are variably regulated in bacteria, and in contrast to B. subtilis, rsbU in EGDe Delta sigB may compensate for the r B - dependent genes that are necessary for tolerance to rifampin stress and therefore plays a role in overcoming the antibiotic- triggered growth arrest.