Biochemical and Biophysical Research Communications, Vol.424, No.2, 338-340, 2012
Evidence of a direct cellular protective effect of Rho-kinase inhibitors on endothelin-induced cardiac myocyte hypertrophy
Using a cellular approach, the present study examined whether fasudil and active metabolite hydroxy-fasudil, Rho-kinase inhibitors, exert a direct protective effect on endothelin-induced cardiac myocyte hypertrophy in vitro. Treatment with endothelin (10 nM) caused significant hypertrophy of cultured neonatal rat cardiomyocytes by a 21.2% increase in cell surface area. Fasudil (1-10 mu M) and hydroxyfasudil (0.3-10 mu M) significantly prevented endothelin-induced cardiomyocyte hypertrophy. The present results suggest that inhibition of cardiac hypertrophy by fasudil is, at least in part, due to direct protection of cardiomyocytes from hypertrophy. (C) 2012 Elsevier Inc. All rights reserved.