Journal of the American Chemical Society, Vol.134, No.17, 7235-7237, 2012
Supramolecular Displacement-Mediated Activation of a Silent Fluorescence Probe for Label-Free Ligand Screening
We report a new approach for the rapid screening of analyte binding affinities for a target protein. We demonstrate that a molecular probe, with a profluorophore substrate and ligand moieties, can be hindered from enzymatic access when bound to the target protein. When analytes displace the probe from the protein's binding pocket, a fluorescence profile is generated. This profile is used to discriminate analytes based on their relative binding affinities.