화학공학소재연구정보센터
Journal of Polymer Science Part A: Polymer Chemistry, Vol.49, No.15, 3241-3247, 2011
Synthesis and Specific Influenza A Virus-Adsorptive Functionality of Alkyl Curdlan Sulfate-Coated Membrane Filter
To develop a biomaterial with an influenza virus-adsorptive functionality, an alkyl curdlan sulfate was prepared by ionic interaction between a positively charged didodecyldimethyl ammonium bromide and a negatively charged sulfate group of curdlan sulfate, which has potent anti-HIV activity, and then it was coated on a membrane filter with a 1-mu m pore size by hydrophobic interaction with the long alkyl groups in the curdlan sulfate. The alkyl curdlan sulfate with the degree of alkylation (DOA) of 0.03 (one didodecyldimethyl group/12 sugar residues with 36 hydroxyl or sulfate groups) showed potent anti-HIV activity in a 50% effective concentration (EC(50)) as low as 0.87 mu g/mL (standard curdlan sulfate EC(50) 0.3 mu g/mL), and the activity decreased with increasing DOA. A DOA higher than 0.1 (one didodecyldimethyl group/three sugar residues with nine hydroxyl or sulfate groups) gave no anti-HIV activity. Although both curdlan sulfate and alkyl curdlan sulfates did not inhibited infection of Madin-Darby canine kidney cells by influenza viruses, the alkyl curdlan sulfate-coated membrane filter was found to have a specific adsorptive functionality for influenza A virus in vitro. When 1.6 mg of the alkyl curdlan sulfate with the DOA of 0.03 was coated on a membrane filter (phi 13 mm; pore size, 1 mu m), three stacked alkyl curdlan sulfate-coated membrane filters dramatically decreased hemagglutination to 1/4-1/32. However, the membrane filter did not effectively remove on influenza B viruses, and thus a membrane filter without alkyl curdlan sulfate was not effective against influenza viruses. These results can therefore be presumed to demonstrate that the alkyl curdlan sulfate-coated membrane filter removed influenza A viruses by adsorption between the negatively charged sulfate groups and the positively charged envelope protein of the virus. (C) 2011 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 49: 3241-3247, 2011