화학공학소재연구정보센터
Journal of Bioscience and Bioengineering, Vol.110, No.3, 374-376, 2010
Generation of single-chain Fvs against detergent-solubilized recombinant antigens with a simple coating procedure
Antigen coating on polystyrene is prevented by detergent. We present here a simple procedure to coat detergent-solubilized antigen for subsequent panning selection of single-chain Fv (scFv), the target antigen of which was the hepatitis C virus (HCV) non-structural protein (NS) 4B, an integral membrane protein. (C) 2010, The Society for Biotechnology, japan. All rights reserved.