화학공학소재연구정보센터
Chemical Physics Letters, Vol.500, No.4-6, 323-326, 2010
Terbium chelate as donor label in time-resolved fluorescence energy transfer study of DNA hybridization
A terbium chelate formed with diethylenetriaminepentaacetic acid and p-aminosalicylic acid (Tb-DTPA-pAS) was labeled on a 23-mer DNA strand and used as donor in the fluorescence resonance energy transfer (FRET) study of DNA hybridization. The change in donor fluorescence lifetime with and without DNA-bind acceptor carboxytetramethylrhodamine (TAMRA) showed high energy transfer efficiency (87%). The distance between the two ends of the 23-mer double-stranded DNA was estimated. This study provides a possibility of using the Tb-chelate as a probe in the studies of the geometry-dependent interaction between DNA strands or proteins. (C) 2010 Elsevier B.V. All rights reserved.