화학공학소재연구정보센터
Chemical Engineering & Technology, Vol.34, No.2, 241-248, 2011
Supercritical CO2 Extraction of Chlorophyll a from Spirulina platensis with a Static Modifier
Supercritical CO2 extraction with a static modifier was applied to extract chlorophyll a from Spirulina platensis. The effects of the process were investigated by single-factor and response surface analysis experiments. The optimal process parameters for supercritical CO2 extraction were determined to be: ethanol/water as the modifier, 40 vol.-% water content in the modifier, 21.2 mL modifier volume, 1 h static soaking time, 2 h dynamic extraction time, 48.7 MPa extraction pressure, 326.4 K extraction temperature, and 10 g CO2 flow rate. The optimized chlorophyll a extraction yield was 6.84 mg g(-1). A comparison of the experimental results suggested that the yield of chlorophyll a by supercritical CO2 extraction with modifier was higher than that obtained by conventional solvent extraction.