화학공학소재연구정보센터
Biotechnology Progress, Vol.27, No.3, 698-705, 2011
A Novel Synthesis of Iminodiacetic Acid: Biocatalysis by Whole Alcaligenes faecalis ZJB-09133 Cells from Iminodiacetonitrile
Iminodiacetic acid (IDA) has been widely used as an important intermediate in the fine chemical industry. In this study, a novel synthesis route of IDA from iminodiacetonitrile by whole microorganisms was investigated. A strain with the capability of producing nitrilase, ZJB-09133, was isolated and identified, and later named Alcaligenes faecalis ZJB-09133. In addition, the detailed biocatalysis of iminodiacetonitrile to produce IDA using ZJB-09133 was investigated. The results showed that the conversion reached 65.3% in Na2HPO4-NaH2PO4 buffer of pH 8.0 under the following conditions: cells in the amount of 0.075-g DCW/L, 1.5% substrate, conversion time of 8 h, and a reaction temperature of 35 degrees C. To the best of our knowledge, this is the first time that the production of IDA using a biocatalysis method has been reported. (C) 2011 American Institute of Chemical Engineers Biotechnol. Prog., 27: 698-705, 2011