화학공학소재연구정보센터
Chemical Physics Letters, Vol.493, No.1-3, 165-169, 2010
A kinetic analysis of strand breaks on large DNA induced by cigarette smoke extract
We report a kinetic analysis of strand breakages on large DNA molecules induced by cigarette smoke extract (CSE), an extract of soluble cigarette smoke components. Previously, this DNA damage was analyzed by agarose gel electrophoresis, whereas we used fluorescence to kinetically analyze damage to individual DNA molecules. CSE caused a marked change in length of DNA molecules. The rate of CSE-induced double-strand breakage on large random-coiled DNA molecules was determined using a simple theoretical model, allowing the facile estimation of the rate of double-strand breaks on large DNA molecules. (C) 2010 Elsevier B. V. All rights reserved.