화학공학소재연구정보센터
Chemical Engineering & Technology, Vol.33, No.1, 52-62, 2010
Glutaminase Production using Zygosaccharomyces rouxii NRRL-Y 2547: Effect of Aeration, Agitation Regimes and Feeding Strategies
Systematic investigations on the effect of varying dissolved oxygen (DO) concentrations and agitation profiles on glutaminase yield from Zygosaccharomyces rouxii NRRL Y-2547 were conducted in a batch stirred tank reactor. The agitation rate was varied in the range of 100-400 rpm, while the DO values studied were 20, 40 and 60%, respectively. Furthermore, a stepwise reduction in DO was investigated wherein the DO concentration was decreased from 40% for the first 12 h to 20% for the following 24 h, and finally decreased to 5% DO up to 54 h (end of batch period). The maximum enzyme yield in A cases was observed after 48 h at 37 degrees C. An agitation rate of 200 rpm and a three-stage DO maintenance strategy (40-20-5% DO) gave maximum glutaminase productivity. Yeast extract and glucose feeding increased the enzyme production from 370.75 +/- 4.97 units/L to 437.14 +/- 4.44 and 417.99 +/- 5.82 units/L, respectively.