Biotechnology and Bioengineering, Vol.106, No.2, 319-325, 2010
Integrated Single-Cell Analysis Shows Pichia pastoris Secretes Protein Stochastically
The production of heterologous proteins by secretion from cellular hosts is an important determinant for the cost of biotherapeutics. A single-cell analytical method called microengraving was used to examine the heterogeneity in secretion by the methylotrophic yeast Pichia pastoris. We show that constitutive secretion of a human Fc fragment by P. pastoris is not cell-cycle dependent, but rather fluctuates between states of high and low productivity in a stochastic manner. Biotechnol. Bioeng. 2010;106: 319-325. (C) 2010 Wiley Periodicals, Inc.