Biochemical and Biophysical Research Communications, Vol.391, No.1, 152-158, 2010
Differential expression of P-type ATPases in intestinal epithelial cells: Identification of putative new atp1a1 splice-variant
P-type ATPases are membrane proteins that couple ATP hydrolysis with cation transport across the membrane. Ten different subtypes have been described. In mammalia, 15 genes of P-type ATPases from subtypes II-A, II- B and II-C, that transport low-atomic-weight cations (Ca2+, Na+, K+ and H+). have been reported. They Include reticulum and plasma-membrane Ca+-ATPases, Na+/K+-ATPase and H+/K+-ATPases. Enterocytes and colonocytes show functional differences. which seem to be partially due to the differential expression of P-type ATPases. These enzymes have 9 structural motifs. being the phosphorylation (E) and the Mg(2+)ATP-binding(H) motifs the most preserved These structural characteristics permitted developing a Multiplex-Nested-PCR (MN-PCR) for the simultaneous identification of different P-type ATPases. Thus, using MN-PCR, seven different cDNAs were cloned from enterocytes and colonocytes, including SERCA3, SERCA2, Na+/K+-ATPase alpha 1-isoform. H+/K+-ATPase alpha 2-isoform, PMCA1, PMCA4 and a cDNA-fragment that seems to be a new cassette-type splice-variant of the atp1a1 gen. PMCA4 in enterocytes and H+/K+-ATPase alpha 2-isoform in colonocytes were differentially expressed This cell-specific expression pattern is related with the distinctive enterocyte and colonocyte functions (c) 2009 Elsevier Inc. All rights reserved.