화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.390, No.3, 673-677, 2009
Overexpression of IbpB enhances production of soluble active Streptomyces olivaceovirdis XynB in Escherichia coli
The small heat shock protein lbpB of Escherichia coli can accelerate protein disaggregation from inclusion body by Hsp100-Hsp70 re-activation system in vitro. It was therefore hypothesized that overexpression of IbpB might be able to promote protein disaggregation from inclusion body, by which more soluble recombinant proteins would be obtained. The overexpression of lbpB actually enhanced production of more active soluble XynB of Streptomyces olivaceovirdis in E. coli BL21 (DE3). Surprisingly, the disaggregation of XynB from inclusion body was not accelerated. It seemed that the overexpressed IbpB protected improperly or partially folded XynB from aggregation and mediated the subsequent refolding. These results show potential of improving production of active heterologous proteins in E. coli. (C) 2009 Elsevier Inc. All rights reserved.