화학공학소재연구정보센터
Journal of Industrial and Engineering Chemistry, Vol.16, No.1, 119-121, January, 2010
Characterization of an inclusion complex of, 7-dehydrocholesterol and cyclodextrin
E-mail:
7-Dehydrocholesterol (7-DHC) plays an important role in the biosynthesis of vitamin D. It is virtually insoluble in water, in which its manipulation is difficult, but the problems may be alleviated by cyclodextrin inclusion. Cyclodextrins are known to form inclusion complex with many kinds of hydrophobic compounds because theirmolecular structure generates a hydrophilic exterior surface and a hydrophobic cavity interior. The purpose of this study was to characterize inclusion complex of 7-dehydrocholesterol with cyclodextrin (hydroxypropyl-β-cyclodextrin, HPβCD). The inclusion complex showed good solubility in contrast to physical mixture. The inclusion efficiency determined by HPLC analysis was 83.72%. Additionally, the inclusion complex was characterized by UV-vis spectrophotometry, Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC). These results demonstrated that 7-dehydrocholesterol with hydroxypropyl-β-cyclodextrin formed an inclusion complex.
  1. Duchene D, Wouessidjewe D, Drug Dev. Ind. Pharm., 16, 2487 (1990)
  2. Jeong YI, Kim DG, Seo DH, Jang MK, Nah JW, J. Ind. Eng. Chem., 14(6), 747 (2008)
  3. Loftsson T, Brewster ME, J. Pharm. Sci., 85, 1017 (1996)
  4. Martin Del Valle EM, Process Biochem., 39, 1033 (2004)
  5. Atwood JL, Davis JED, MacNicol DD, Vogtle F, Lehn JM, Szejtli J, Osa T, Comprehensive Supramolecular Chemistry, vol. 3, Elsevier Science, New York (1996)
  6. Albers E, Muller BW, J. Pharm. Sci., 81, 756 (1992)
  7. Brewster ME, Estes KS, Bodor N, Int. J. Pharm., 59, 231 (1990)
  8. Cho SH, Kim SY, Lee SI, Lee YM, J. Ind. Eng. Chem., 12(1), 50 (2006)
  9. Yoo JW, Kim TY, Cho SY, Rho SG, Kim SJ, J. Ind. Eng. Chem., 10(2), 257 (2004)
  10. Anthony NW, Am. J. Clin. Nutr., 67, 1108 (1998)
  11. Botella SM, Martin MA, Del Castillo B, Menendez JC, Vazquez L, Lerner DA, J. Pharm. Biomed. Anal., 14, 909 (1996)
  12. Williams III RO, Mahaguna V, Sriwongjanya M, Eur. J. Pharm. Biopharm., 46, 355 (1998)