화학공학소재연구정보센터
Applied Chemistry for Engineering, Vol.21, No.1, 40-45, February, 2010
소수성 플라보노이드인 루테올린을 함유한 Ethosome의 제조 및 특성조사
Preparation and Characterization of Ethosome Containing Hydrophobic Flavonoid Luteolin
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초록
소수성 플라보노이드인 루테올린의 안정성을 향상시키고 또한 의약이나 화장품 분야에 널리 활용하기 위해서 ethosome에 루테올린 포집을 시도하였다. 편광현미경과 열분석기인 DSC 등을 사용하여 액정의 형성과정이나 열적 특성 등을 관찰하였다. 에탄올에 용해된 레시틴 혼합물에 PBS 완충용액 첨가에 따른 W/O에서 W/O/W로의 상 전환 시점은 전도도 변화로 관측될 수 있었다. Ethosome을 구성하는 성분 및 조성에 따라 입자크기가 달라지는데 레시틴에 루테올린을 10% 정도 혼합하여도 입자크기에 별 영향이 없었다. 루테올린이 ethosome에 포집되었을 때 안정성이 향상되는 효과를 DPPH 실험을 통해 확인하였다. Ethosome의 안정화 효능은 토코페롤 첨가에 의해 더욱 향상되었다.
Entrapment of hydrophobic flavonoid luteolin into ethosome was carried out for improving its stability and making practical application in the field of drug and cosmetics. The formation of liquid crystalline phase and its thermal properties were investigated by polarized optical microscopy and DSC. The phase inversion from W/O to W/O/W was detected by conductivity change with the addition of PBS buffer solution into the ethanol-dissolved lecithin mixture. The particle size change of ethosome with constituent composition was examined, which showed that the incorporation of luteolin into lecithin up to 10% had little effect on the size of ethosome. Enhancement of stability of luteolin by entrapment into ethosome was verified through DPPH test. The stabilization efficacy of ethosome was improved further by the addition of tocopherol.
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