화학공학소재연구정보센터
Journal of Bioscience and Bioengineering, Vol.108, No.4, 282-285, 2009
Expression and purification of the recombinant mustard trypsin inhibitor 2 (MT12) in Escherichia coli
The mustard trypsin inhibitor 2, MT12, was expressed in Escherichia coli. A specific procedure for its production and purification is described. The recombinant protein was recovered by protein extraction from the insoluble fraction, then renatured and purified by ion exchange and gel filtration chromatography. Finally, the inhibitory activity against trypsin was also determined. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.