화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.377, No.4, 1047-1050, 2008
Establishment of a new glial cell line from hippocampus of prion protein gene-deficient mice
Cellular prion protein (PrPC) is expressed not only in neuronal cells but also in non-neuronal cells such as astroglial cells. In the present study, the prion protein (PrP) gene (Prnp)-deficient astroglial cell line GpL1 from hippocampal cells of Zrchl Prnp(-/-) mice were established. Transfection of Prnp suppressed cell death in GpL1 cells under serum-free conditions. The PrP-expressing GpL1 cells showed increased superoxide dismutase activity compared to control GpL1 cells. These results suggest that the anti-oxidative activity of PrPC functions in not only neuronal cells but also astroglial cells possibly due to the increased anti-oxidative activity of astroglial cells. (C) 2008 Elsevier Inc. All rights reserved.