화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.375, No.3, 346-350, 2008
Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30/GNL knockout mice
Hydrogen is an established anti-oxidant that prevents acute oxidative Stress. To Clarify the mechanism of hydrogen's effect in the brain, we administered hydrogen-rich pure water (H-2) to senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice. which cannot synthesize vitamin C (VC) also a well-known anti-oxidant. These KO mice were divided into three groups: recipients of H-2, VC, or pure water (H2O), administered for 33 days. VC levels in H-2 and H2O groups were <6% of those in the VC group. Subsequently, superoxide formation during hypoxia-reoxygenation treatment of brain slices from these groups was estimated by a real-time biography imaging system, which models living brain tissues, with Lucigenin used as chemiluminescence probe for superoxide. A significant 27.2% less superoxide formed in the H-2 group subjected to ischemia-reperfusion than in the H2O group. Thus hydrogen-rich pure water acts as an anti-oxidant in the brain slices and prevents superoxide formation. (C) 2008 Elsevier Inc. All rights reserved.