화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.366, No.4, 1025-1029, 2008
Low temperature induces the delivery of mature and immature CFTR to the plasma membrane
Deletion of phenylalanine 508 (Delta F508) is the most prevalent disease-causing mutation resulting in retention of the immature CFTR in the endoplasmic reticulum. The most common strategy to induce the delivery of Delta F508-CFTR to the surface of cells is by reducing the incubation temperature (approximate to 28 degrees C). Cell surface biotinylation of HEK293T cells grown at 37 degrees C for 48 h, confirmed the presence of mature wild-type CFTR, but not AF508-CFTR at the cell surface. On the other hand, cells incubated at 28 degrees C for 16 h showed both mature and immature Delta F508-CFTR at their surface. The trafficking of immature Delta F508-CFTR, but not mature Delta F508-CFTR, to the cell surface occurred at low temperature even upon addition of BFA, suggesting the involvement of a Golgi-independent pathway. These results suggest that low temperature induces the appearance of a mix population of mature and immature CFTR molecules at the plasma membrane through distinct pathways. (c) 2007 Elsevier Inc. All rights reserved.