화학공학소재연구정보센터
Biomacromolecules, Vol.8, No.12, 3959-3964, 2007
Chemoenzymatic syntheses of amylose-grafted chitin and chitosan
Amylose-grafted chitin and chitosan were synthesized by chemoenzymatic methods according to the following reaction manners. First, maltoheptaose was introduced to chitosan by a reductive amination using sodium cyanotrihydroborate in a mixed solvent of 1.0 mol/L aqueous acetic acid and methanol at room temperature to produce a maltoheptaose-grafted chitosan (1). The functionality of maltoheptaose to chitosan in 1 depended on reaction time. The phosphorylase-catalyzed enzymatic polymerization of a-D-glucose 1-phosphate was then performed from 1 to obtain amylose-grafted chitosan (2). Mahoheptaose-grafted chitin (3) was synthesized by N-acetylation of 1 using acetic anhydride in a mixed solvent of aqueous acetic acid and methanol. Then, synthesis of amylose-grafted chitin (4) was performed by the phosphorylase-catalyzed enzymatic polymerization under conditions the same as those for 2. The average DPs of amylose graft chains in 2 and 4 depended on the feed ratios of a-D-glucose 1-phosphate to mahoheptaose primers in 1 and 3.