화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.362, No.1, 170-176, 2007
Centrin controls the activity of the ciliary reversal-coupled volt-age-gated Ca2+ channels Ca2+-dependently
In Paramecium, ciliary reversal is coupled with voltage-gated Ca2+ channels on the ciliary membrane. We previously isolated a P. caudatum mutant, cnrC, with a malfunction of the Ca2+ channels and discovered that the channel activity of cnrC was restored by transfection of the P. caudatum centrin (Pccentrin1p) gene, which encodes a member of the Ca2+-binding EF-hand protein family. In this study, we injected various mutated Pecentrin1p genes into cnrC and investigated whether these genes restore the Ca2+ channel activity of cnrC. A Pccentrin1p mutant gene lacking Ca2+ sensitivity of the third and fourth EF-hands lost the ability to restore the channel function of cnrC, and mutation of the fourth EF-hand caused more serious impairment than mutation of the third EF-hand. Moreover, a Pccentrin1p gene lacking the N-terminal 34-amino acid sequence also lost the ability to restore the channel activity. Native-PAGE analysis demonstrated that the N-terminal sequence is important for the Ca2+-dependent structural change of Pccentrin1p. These results demonstrate that Pccentrin1p Ca2+-dependently regulates the Ca2+ channel activity in vivo. (C) 2007 Elsevier Inc. All rights reserved.