화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.361, No.4, 1017-1021, 2007
Isw2 regulates gene silencing at the ribosomal DNA locus in Saccharomyces cerevisiae
Three heterochromatin-like domains have been identified in Saccharomyces cerevisiae that are refractory to transcription by Pol II, the silent mating-type loci, telomeres and the ribosomal DNA. Previous work has shown that chromatin remodelers can regulate silent chromatin. Here, we report the findings of an investigation into the role of ISW2 in transcriptional silencing at the rDNA. We show that the levels of retrotransposition and mRNA from a genetically marked Tyl element located in the rDNA were increased significantly in isw2 Delta cells, while transcript levels from Tyl elements outside of the rDNA were not increased in cells lacking ISW2. Additionally, we show that Isw2 is not required for silencing at a telomere. Our findings demonstrate that Isw2 is required for transcriptional silencing at the rDNA and emphasize the differences in the regulation of transcriptional silencing at silent loci in S. cerevisiae. (C) 2007 Elsevier Inc. All rights reserved.