화학공학소재연구정보센터
Macromolecular Research, Vol.16, No.2, 139-148, February, 2008
Preparation and Characterization of Temperature-Sensitive Poly(N-isopropylacrylamide)-g-Poly(L-lactide-co-ε-caprolactone) Nanofibers
E-mail:
Biodegradable and elastic poly(L-lactide-co-ε-caprolactone) (PLCL) was electrospun to prepare nanofibers, and N-isopropylacrylamide (NIPAAm) was then grafted onto their surfaces under aqueous conditions using 60Co-γ irradiation. The graft yield increased with increasing irradiation dose from 5 to 10 kGy and the nanofibers showed a greater graft yield compared with the films. SEM confirmed that the PLCL nanofibers maintained an interconnected pore structure after grafting with NIPAAm. However, overdoses of irradiation led to the excessive formation of homopolymer gels on the surface of the PLCL nanofibers. The equilibrium swelling and deswelling ratio of the PNIPAAm-g-PLCL nanofibers (prepared with 10 kGy) was the highest among the samples, which was consistent with the graft yield results. The phase-separation characteristics of PNIPAAm in aqueous conditions conferred a unique temperature-responsive swelling behavior of PNIPAAm-g-PLCL nanofibers, showing the ability to absorb a large amount of water at < 32 ℃, and abrupt collapse when the temperature was increased to 40 ℃. In accordance with the temperature-dependent changes in swelling behavior, the release rate of indomethacin and FITC-BSA loaded in PNIPAAm-g-PLCL nanofibers by a diffusion-mediated process was regulated by the change in temperature. Both model drugs demonstrated greater release rate at 40 ℃ relative to that at 25 ℃. This approach of the temperature- controlled release of drugs from PNIPAAm-g-PLCL nanofibers using gamma-ray irradiation may be used to design drugs and protein delivery carriers in various biomedical applications.
  1. Jayaraman K, Kotaki M, Zhang YZ, Mo XM, Ramakrishna S, J. Nanosci. Nanotechnol., 4, 52 (2004)
  2. Reneker DH, Yarin AL, Zussman E, Xu H, Adv. Appl. Mech., 41, 43 (2007)
  3. Courtney T, Sacks MS, Stankus J, Guan J, Wagner WR, Biomaterials, 27, 3631 (2006)
  4. Nair LS, Bhattacharyya S, Laurencin CT, Expert Opin. Biol. Ther., 4, 659 (2004)
  5. Goldberg M, Langer R, Jia XQ, J. Biomater. Sci.-Polym. Ed., 18, 241 (2007)
  6. Jing Z, Xu XY, Chen XS, Liang QZ, Bian XC, Yang LX, Jing XB, J. Control. Release, 92, 227 (2003)
  7. Kim K, Luu YK, Chang C, Fang DF, Hsiao BS, Chu B, Hadjiargyrou M, J. Control. Release, 98, 47 (2004)
  8. Ma ZW, Kotaki M, Inai R, Ramakrishna S, Tissue Eng., 11, 101 (2005)
  9. Venugopal J, Ramakrishna S, Appl. Biochem. Biotechnol., 125(3), 147 (2005)
  10. Zhang YZ, Wang X, Feng Y, Li J, Lim CT, Ramakrishna S, Biomacromolecules, 7(4), 1049 (2006)
  11. Takezawa T, Biomaterials, 24, 2267 (2003)
  12. Jeon EK, Whang HJ, Khang G, Lee I, Rhee JM, Lee HB, Polym.(Korea), 25(6), 893 (2001)
  13. Kroese-Deutman HC, Ruhe PQ, Spauwen PHM, Jansen JA, Biomaterials, 26, 1131 (2005)
  14. Sohier J, Haan RE, de Groot K, Bezemer JM, J. Control. Release, 87, 57 (2003)
  15. Yarlagadda PKDV, Chandrasekharan M, Shyan JYM, Bio-Med. Mater. Eng., 15, 159 (2005)
  16. Zhang YF, Cheng XR, Wang JW, Wang YN, Shi B, Huang C, Yang XC, Liu TJ, Biochem. Biophys. Res. Commun., 344(1), 362 (2006)
  17. Xu XD, Wei H, Zhang XZ, Cheng SX, Zhuo RX, J. Biomed. Mater. Res. A, 81A, 418 (2007)
  18. Zhang XZ, Lewis PJ, Chu CC, Biomaterials, 26, 3299 (2005)
  19. Ju HK, Kim SY, Lee YM, Polymer, 42(16), 6851 (2001)
  20. Kim JH, Lee SB, Kim SJ, Lee YM, Polymer, 43(26), 7549 (2002)
  21. Kim SY, Cho SM, Lee YM, Kim SJ, J. Appl. Polym. Sci., 78(7), 1381 (2000)
  22. Yoo MK, Sung YK, Lee YM, Cho CS, Polymer, 41(15), 5713 (2000)
  23. Zhai ML, Liu N, Li J, Yi M, Li JQ, Ha HF, Radiat. Phys. Chem., 57, 481 (2000)
  24. Zhang H, Chu LY, Li YK, Lee YM, Polymer, 48(6), 1718 (2007)
  25. Cho EH, Lee SG, Kim JK, Curr. Appl. Phys., 5(5), 475 (2005)
  26. Shim JK, Na HS, Lee YM, Huh H, Nho YC, J. Membr. Sci., 190(2), 215 (2001)
  27. Yang Y, Porte MC, Marmey P, El Haj AJ, Amedee J, Baquey C, Nucl. Instrum. Methods Phys. Res. Sect. B-Beam Interact. Mater. Atoms, 207, 165 (2003)
  28. Zhang CH, Huang YD, Zhao YD, Mater. Chem. Phys., 92(1), 245 (2005)
  29. Haugen H, Gerhardt LC, Will J, Wintermantel E, J. Biomed. Mater. Res. B, 73B, 229 (2005)
  30. Haugen HJ, Brunner M, Pellikofer F, Aigner J, Will J, Wintermantel E, J. Biomed. Mater. Res. B, 80B, 415 (2007)
  31. Lin FH, Chen TM, Chen KS, Wu TH, Chen CC, Mater. Chem. Phys., 64(3), 189 (2000)
  32. Shariatpanahi RE, Orang F, Emami SH, Naimi T, J. Biomater. Sci.-Polym. Ed., 17, 659 (2006)
  33. Dunn RL, Yewey GL, Fujita SM, Josephs KR, Whitman SL, Southard GL, Dernell WL, Straw RC, Withrow SJ, Powers BE, J. Bioact. Compat. Polym., 11, 286 (1996)
  34. Jeong SI, Kwon JH, Lim JI, Cho SW, Jung YM, Sung WJ, Kim SH, Kim YH, Lee YM, Kim BS, Choi CY, Kim SJ, Biomaterials, 26, 1405 (2005)
  35. Zhu YB, Leong MF, Ong WF, Chan-Park MB, Chian KS, Biomaterials, 28, 861 (2007)
  36. Jeong SI, Kim BS, Lee YM, Ihn KJ, Kim SH, Kim YH, Biomacromolecules, 5(4), 1303 (2004)
  37. Jeong SI, Kim SH, Kim YH, Jung Y, Kwon JH, Kim BS, Lee YM, J. Biomater. Sci.-Polym. Ed., 15, 645 (2004)
  38. Arenas E, Bucio E, Burillo G, Lopez GP, Polym. Bull., 58(2), 401 (2007)
  39. Lee SB, Park EK, Lim YM, Cho SK, Kim SY, Lee YM, Nho YC, J. Appl. Polym. Sci., 100(6), 4439 (2006)
  40. Liu Q, Zhu ZY, Yang XM, Chen XL, Song YF, Radiat. Phys. Chem., 76, 707 (2007)
  41. Lee SB, Seo SM, Lim YM, Cho SK, Lee YM, Macromol. Res., 12(3), 269 (2004)
  42. Grondahl L, Cardona F, Chiem K, Wentrup-Byrne E, J. Appl. Polym. Sci., 86(10), 2550 (2002)
  43. Kimura S, Yasuda K, Hara N, Sakai T, Mikami S, Minami A, Tohyama H, Arthroscopy, 19, 68 (2003)
  44. Kesenci K, Motta A, Fambri L, Migliaresi C, J. Biomater. Sci.-Polym. Ed., 12, 337 (2001)
  45. Liu SQ, Yang YY, Liu XM, Tong YW, Biomacromolecules, 4(6), 1784 (2003)
  46. Bucio E, Arenas E, Burillo G, Mol. Cryst. Liq. Cryst., 447, 521 (2006)
  47. Peng T, Cheng YL, J. Appl. Polym. Sci., 70(11), 2133 (1998)
  48. Kaneko Y, Yoshida R, Sakai K, Sakurai Y, Okano T, J. Membr. Sci., 101(1-2), 13 (1995)
  49. Yoshida R, Uchida K, Kaneko Y, Sakai K, Kikuchi A, Sakurai Y, Okano T, Nature, 374(6519), 240 (1995)
  50. Kim JH, Lee TR, Langmuir, 23(12), 6504 (2007)
  51. Cui WG, Li XH, Zhu XL, Yu G, Zhou SB, Weng J, Biomacromolecules, 7(5), 1623 (2006)