Biochemical and Biophysical Research Communications, Vol.302, No.2, 392-396, 2003
Carbon monoxide exposure in rat heart: glutathione depletion is prevented by antioxidants
Rat hearts were perfused for 15 min with buffer equilibrated with 0.01% or 0.05% CO. The buffer was equilibrated with 21% O-2 throughout. The ventricular glutathione content decreased by 76% and 84%, 90 min post-exposure to 0.01% and 0.05% CO, respectively, compared with 0% CO controls (0.45 +/- 0.01 mumol/g wet tissue; +/-SEM, n = 3). Both reduced and oxidised glutathione contributed to this decline. When ascorbate and Trolox C were included during exposure to 0.05% CO the glutathione pool was partly protected; here the glutathione decrease was 46%. In most hearts additional creatine kinase activity in the perfusate indicated minor tissue injury occurring immediately after the start and/or about 10 min after the end of exposure to 0.01% CO or 0.05% CO. Ventricle lactate levels were unaffected by exposure to 0.01% CO. This evidence supports a role for oxidative stress in CO cardiotoxicity. (C) 2003 Elsevier Science (USA). All rights reserved.