화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.301, No.4, 1122-1128, 2003
PKC regulates the delta 2 glutamate receptor interaction with S-SCAM/MAGI-2 protein
inside cells, membrane proteins are localized at particular surface domains to perform their precise functions. Various kinds of PDZ domain proteins have been shown to play important roles in the intracellular trafficking and anchoring of membrane proteins. In this study, we show that 62 glutamate receptor is interacting with S-SCAM/MAGI-2, a PDZ domain protein localized in the perinuclear region and postsynaptic sites of cerebellar Purkinje cells. The binding is regulated by PKC (protein kinase-C) mediated phosphorylation of the receptor with a unique repetitive structure in S-SCAM/MAGI-2. Co-expression of both proteins resulted in drastic changes of the receptor localization in COS7 cells. These results show a novel regulatory mechanism for the binding of PDZ domain proteins and suggest that the interaction between 62 receptor and S-SCAM/MAGI-2 may be important for intracellular trafficking of the receptor. (C) 2003 Elsevier Science (USA). All rights reserved.